Single Nucleotide Polymorphisms Marks of Coccinia grandis L., in the Mekong Delta

Do Mai, Thieu Duong and Mai Hung

Background and Objective: Despite its ecological and agricultural importance, Coccinia grandis remains understudied in the Mekong Delta, particularly regarding morphological diversity and genetic variation. This study aims to analyze the species’ morphological characteristics and genetic diversity using SNP markers. Specifically, it seeks to assess variations in morphological traits among different populations and evaluate genetic diversity and relationships through SNP analysis to better understand its population structure. Materials and Methods: The eight samples of Coccinia grandis L., the method of observing and describing the external morphology and microsurgery of Coccinia grandis L., was carried out based on the botanical research method with improvements to suit the experimental conditions. The quality of the DNA was checked by electrophoresis on a 1% agarose gel using safe-view dye. Results: The stem height, leaf length and root length show that there are ecological and nutritional influences leading to the above morphological differences. The results of comparing the sequences of 8 Coccinia grandis L., varieties on the NCBI GenBank show that the samples are similar to the sequence of the species Coccinia grandis L. Conclusion: With molecular biology techniques, it has been determined that all 8 Coccinia grandis L., varieties belong to the species Coccinia grandis L. From there, it can be applied to the pharmaceutical production industry to be more diversified.

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