Background and Objective: Dengue cases have increased while the spread is getting broader worldwide. Temephos has been frequently used to control the larvae of the Aedes aegypti L., the primary vector of dengue. The intensive use of this larvicide has given rise to resistance. This study aims to determine the susceptibility status of Ae. aegypti to temephos and examine the two mutations (F290V and F455W) that possibly occur in the Ace-1 gene of Ae. aegypti from Salido Sub-District, IV Jurai District, Pesisir Selatan Regency. Materials and Methods: The susceptibility test was performed referring to a standard method of the World Health Organization, followed by a molecular test (polymerase chain reaction) and sequencing. Results: The results showed that the larvae of Ae. aegypti have been tolerant to temephos (0.012 mg L1) with a percentage of larval mortality of 91.67%. The sequencing analysis in the Ace-1 gene revealed the absence of F290V and F455W mutation in temephos-resistant Ae. aegypti, but a point mutation was detected at codon 506. This mutation shifts the ACA codon to ACT, but still codes for the same amino acid, threonine. Conclusion: Our study indicates the presence of other resistance mechanisms in the major dengue vector of the Salido District. Implementation of the alternative population control strategy is required to prevent the temephos resistance further.
Resti Rahayu, Defrian Melta and Hasmiwati, 2022. Detection of Ace-1 Mutation in Temephos-Resistant Aedes aegypti L. in West Sumatra, Indonesia. Pakistan Journal of Biological Sciences, 25: 816-821.