Analysis of Apoptotic Cells and Lung Inflammation after Given by Vitis gracilis

Nila Wasnis, Syafruddin Ilyas, Salomo Hutahaean, Ramlan Silaban and Putri Situmorang

Background and Objective: Herbs have the potential to be used in molecular therapy to treat inflammation and apoptosis. It has been demonstrated that the Indonesian native Vitris gracilis inhibits cytochrome c as a precursor to apoptosis. When body tissues are injured, infected with bacteria, exposed to toxins or exposed to heat that causes cell death, inflammation occurs. The current study aimed to look for the effect of Vitis gracilis extract in the lung histology of mice. Materials and Methods: Five treatment groups were used for the experiments, which were conducted inside the study. The T1 is the negative control, T2 is swimming mice with 0.2 mg kg^–1 b.wt., of vitamin C, T3 is swimming mice with 50 mg kg^–1 b.wt., of Vitis gracilis, T4 is swimming mice with 75 mg kg^–1 b.wt., of Vitis gracilis and T5 is swimming mice with 100 mg kg^–1 b.wt., of Vitis gracilis. The dislocation procedure was used to dissect mice, ketamine was administered before dissection and the lungs were removed for TUNEL assay examination. Results: There were no significant differences in inflammatory cells or index-positive apoptotic cells between the T1, T2 and T5 groups (p>0.05). The T3 group had the highest value, while the T1 group had the lowest. The highest dose of Vitis gracilis reduced lung cell inflammation while also improving histological structure, resulting in intact, nucleated and complete alveolar membranes with proportional endothelial cells. Conclusion: Vitis gracilis 100 mg kg^–1 b.wt., can repair and reduce inflammation in lung tissue. As a result, the higher the dose of Vitis gracilis, the less cell apoptosis occurs in the lungs.

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