Correlation of Myc Expression with Wee1 Expression by Zanthoxylum acanthopodium in Cervical Carcinoma Histology

Syafruddin Ilyas, Rostime Simanullang, Salomo Hutahaean, Rosidah Rosidah and Putri Situmorang

Background and Objective: Natural herbs and molecular therapy can be used to treat cervical cancer. The Myc and Wee1 control tumour cell fate and microenvironmental changes like angiogenesis activation and host immune response suppression. The study aims to know about the correlation of Myc and Wee1 expressions as a molecular therapy given by Zanthoxylum acanthopodium. Materials and Methods: There are five rat groups: Group K^– is the untreated group, Group K⁺ is the rats injected with benzopyrene, Group P₁ is the administration of Zanthoxylum acanthopodium 100 mg kg^–1 b.wt., Group P₂ is the administration of Zanthoxylum acanthopodium 200 mg kg^–1 b.wt. and Group P₃ is the administration of Zanthoxylum acanthopodium 400 mg kg^–1 b.wt. The rats are dissected 30 days after receiving Zanthoxylum acanthopodium. To stain the cervical tissues, immunohistochemistry is performed. Results: Zanthoxylum acanthopodium administration caused epithelial thickening and decreased Myc expression in previously uncontrolled carcinomas from untreated malignancies, which now slowed and stopped growing into the normal epithelium. Wee1 expression revealed that this herb could repair tissue by drastically reducing Wee1 expression at a dose of 100-400 mg kg^–1 b.wt. Similarly, at the highest dose, cervical carcinoma stops growing and the nucleus begins to form normally (p<0.01). Conclusion: The higher Myc expression on andaliman administration in cervical carcinoma decreases Wee1 expression in cervical carcinoma so these two proteins have a strong and significant correlation. Zanthoxylum acanthopodium can be administered at various dosages to lower the number of positive indexes of Myc and Wee1 expression in cervical carcinoma.

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