Effect of Electricity on the Expression of CGRP and its Receptor RAMP1 in Rat IBS-C Model and its Mechanism of Action

Wei Mao, Fang Xie, Qiong Hu, Ming-Ming Zhang and Xiao-Li Mao

Background and Objective: Irritable Bowel Syndrome (IBS), a common chronic functional disorder, poses a severe risk of increasing physical and mental health concerns. The current animal study was carried out to investigate how electricity affects CGRP and RAMP1 expression and its mode of action. Materials and Methods: In this study, intragastric ice-water techniques were followed. Each group’s rats’ mental state, weight, faeces mass, auricle colour, hair colour and quality, activity level, water and food intake and bowel movement frequency were observed. The leftover small intestine and stomach propulsion of gavaged rats were investigated and ELISA and western blotting were conducted. Results: Rat behaviour before and after treatment was evaluated for each group. The rats in the model and electroacupuncture groups had considerably poorer general condition ratings (p<0.05) than the blank group. In comparison to the blank group, the model rats had a substantial increase (p<0.05) in stomach residual rate and a decrease (p<0.05) in small intestine propulsion rate. Electroacupuncture significantly increased intestinal propulsion rate (p<0.05) and decreased stomach residual rate compared to the model group. Electroacupuncture increased plasma CGRP and SP in both groups. Electroacupuncture significantly altered TRPV1, PAR4, CGRP, RAMP1 and SP mRNA and proteins in all groups. Compared to the blank group, model rats had substantially greater (p<0.05) mRNA and protein expressions of TRPV1, PAR4, CGRP, SP and RAMP1 in their colon tissues. Conclusion: Electroacupuncture regulates TRPV1, PAR4, CGRP, RAMP1 and SP expression, which may help IBS-C stomach pain patients.

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