Effect of Hydrogen Sulfide on Ileum and Colon Motility in Experimental Model of Peritonitis in Rats

Evren Tuncer and Şahin Yildirim

Background and Objective: Studies investigating the association of H₂S and nitric oxide signaling with intestinal motility in peritonitis are limited. The objective of this study was to explore how H₂S influences the motility of the ileum and colon within an experimental peritonitis model. Materials and Methods: Three groups with 6 Wistar albino rats in each were used: Group 1 (Control), Group 2 (Peritonitis) and Group 3 (Sham). The experiment involved inducing contractions using either carbachol or electrical field stimulation (EFS) and relaxation responses are recorded upon the addition of L-cysteine, DL-PAG or NaHS, irrespective of whether L-NAME was added or not. Shapiro-Wilk Francia test, Levene test and Independent -Samples-t tests were used for the statistical analysis. Results: After induction with EFS or after pre-contraction with carbachol, relaxation rate (%) with NaHS was higher than relaxation rates (%) with L-cysteine and DL-PAG in the colon for all groups. The relaxation with NaHS was similar in all ileum groups after induction with EFS (p = 0.073), but in Group 2, responses were higher (p = 0.003 and p<0.001). The L-NAME did reveal a significant change in the presence of NaHS after pre-contraction either with carbachol or EFS in the colon for all groups (p<0.05). Conclusion: The NaHS induced significantly greater relaxation in the colon and it was more effective in the Peritonitis groups. The addition of L-NAME did result in a critical response with NaHS, after pre-contraction with carbachol in the colon Peritonitis group. The well-established fact is that a strong interplay exists between the NO and H₂S signaling pathways.

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