Long Non-Coding RNA MAFG-AS1 Regulates the Proliferation and Epithelial to Mesenchymal Transition of Human Esophageal Carcinoma Cells

Shuhong Kang, Haihua Guo, Ke Lan, Ning Guo, ShiPeng Lu, Feng Lv and Yunfeng Ni

Background and Objective: Esophageal carcinoma (EC) is considered a major type of neoplastic disorder of the human gastrointestinal tract and causes significant mortality and morbidity across the globe. This research was done to find out if the Long Non-coding RNA (lncRNA) MAFG-AS1 affects the growth of human esophageal carcinoma (HEC) cells and their change from an epithelial to a mesenchymal state. Materials and Methods: The 44 pairs of tissue specimens were taken from carcinoma patients between the ages of 38 and 73. The HEC cell lines (EC109, TE-10 and KYSE-410) together with Het-1A and human esophageal epithelial cells, were utilized in this study. Transient transfection, RNA isolation, cDNA synthesis, RT-PCR analysis, MTT proliferation and clonogenic assays, EdU incorporation assays and the transwell assay were performed by standard methods. Results: The outcomes of this investigation showed that EC samples and cell lines exhibit considerable (p<0.05) expression of MAFG-AS1. The MAFG-AS1 knockdown substantially (p<0.05) decreased the growth of EC109 and KYSE-410 esophageal cancer cells via apoptosis, a rise in Bax and a reduction in Bcl-2 in EC109 and KYSE-410 esophageal cancer cells. Furthermore, in EC109 and KYSE-410, ES knockdown enhanced E-cadherin and α-catenin expression while reducing fibronectin and vimentin expression. Conclusion: The MAFG-AS1 can be used as a therapeutic target because it may control how HEC cells grow and change from being epithelial to mesenchymal transition (EMT) cells.

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