Prognostic Values of Tanshinone in Mediating the Activity of Human Umbilical Vein Endothelial Cells

Xiaoyong Tang, Chao Chen, Guangqing Cheng, Yan Chen, Changsheng Li and Xiaoni Yang

Background and Objective: Little is known about the roles of tanshinone in the pathogenesis of disease onset of cardiovascular diseases. In this study, we aimed to identify disease-related genes and analyze prognostic values of tanshinone in the Human Umbilical Vein Endothelial Cells (HUVECs). Materials and Methods: The MTT assay was performed to investigate the cellular availability in the groups including the control group, vehicle group and treatment groups. The tanshinone group was treated with different concentrations of tanshinone. The MTT assay was performed to evaluate the cellular proliferation. Microarray expression profiles of GSE19804 were obtained from the gene expression omnibus (GEO) database. Clustering analysis was utilized to analyze the differentially expressed genes among these groups. The GO and KEGG pathway enrichment was analyzed based upon DEGs. Results: Tanshinone inhibited the proliferation of HUVECs. Tanshinone induced differential expression of genes in vitro. Enrichment analysis indicated that the ERK5 signalling pathway ranked first in the GO and KEGG analysis. Conclusion: Tanshinone played important role in mediating the signalling pathways in the HUVECs. It could induce differentially expressed genes.

View Fulltext Back