Anti-Inflammatory and Immunomodulatory Effects of Hesperidin against the Ovalbumin-Induced Allergic Rhinitis in Mice

Yujuan Liu, Fang Wang, Daning Zhang and Zhaoxia Li

Background and Objective: Allergic Rhinitis (AR), is a nasal mucosal inflammation that arises due to overreacts by the immune system to inhaled allergens and is a common and serious public health problem and affected a large number of the world population. The current experimental study scrutinizes the antiallergic effect of hesperidin against the Ovalbumin (OVA) induced Allergic Rhinitis (AR) in mice via anti-inflammatory and antioxidant parameters. Materials and Methods: Wistar mice were divided into six groups, non-sensitized, ovalbumin sensitized, sensitized treated with hesperidin (5, 10 and 20 mg kg^–1) and montelukast (10 mg kg^–1) for 21 days. On the day of 21, intranasal OVA was induced and end of the experimental protocol spleen weight, biochemical, physiological, Interleukin 4 (IL-4), Interleukin 6 (IL-6), Interleukin 10 (IL-10), Immunoglobulin-E (IgE), Interferon-gamma (IFN-γ), IFN-γ/IL-4 ratio, total protein, phospholipase A₂ (PLA₂) were estimated. However, the mRNA expression of GATA-3, T-bet, FOXP-3 and (ROR)-γt were also estimated. Result: Hesperidin significantly (p<0.001) showed the protection against sneezing, nose redness and nasal rubbing followed nasal challenge. Hesperidin considerably (p<0.001) down-regulated the histamine, nitric oxide production, IL-4, IL-6, IL-10, IgE, TP and PLA₂ as compared to the OVA-induced RA. Hesperidin considerably reduces the ratio of IFN-γ/IL-4 as compared to the OVA-induced RA. Hesperidin considerably down-regulated the mRNA expression of ROR-γt, GATA-3 and up-regulation of FOXP-3 and T-bet expression at concentration-dependent manner. Conclusion: Based on the result, we can conclude that hesperidin may ameliorate the OVA-induced AR condition via alteration of expression of ROR-γt, GATA-3, FOXP-3 and T-bet, which commit T helper cells to TH1 phenotype.

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