Acacetin Improves Renovascular Hypertension Via Inhibition of the Renin-Angiotensin Pathway in Experimental Rats

Zhongying Lv and Wencui Wang

Background and Objective: The kidney is an important organ during blood pressure regulation, however, renal artery occlusion resulted in Renovascular Hypertension (RVH). Researchers have well documented the potential of acacetin against various hypertensive conditions. This study evaluates the putative mechanism of action of acacetin against two-kidney one-clip (2K1C)-induced RVH in experimental rats. Materials and Methods: RVH was induced in male Sprague-Dawley rats by the 2K1C method and then they were treated with vehicle (1% DMSO, 10 mg kg^–1) or captopril (30 mg kg^–1) or acacetin (10, 25 or 50 mg kg^–1) orally for 4 weeks. Results: Occlusion of a renal artery by using 2K1C resulted in marked (p<0.05) alterations in kidney weight, hemodynamic and left ventricular functions in rats which were ameliorated by acacetin (25 or 50 mg kg^–1) treatment. Acacetin also significantly (p<0.05) inhibited 2K1C-induced elevated renal oxido-nitrosative stress and Angiotensin-Converting Enzyme (ACE) activity. Furthermore, acacetin effectively (p<0.05) down-regulated 2K1C-induced up-regulated mRNA expressions of renal KIM-1, NGAL and renin improving renal Ho-1 mRNA expression. In addition, histopathology alteration produced by 2K1C in rat kidneys was ameliorated by acacetin treatment. Conclusion: Administration of acacetin significantly ameliorated elevated renal ACE and renin expression, thus inhibiting renin-angiotensin pathway induced renovascular hypertension in the 2K1C model in rats.

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