Silver/Chitosan/Ascorbic Acid Nanocomposites Attenuates Bacterial Sepsis in Cecal Ligation and Puncture Rat Model

Ayman Saber Mohamed, Saleh Al-Quraishy, Rewaida Abdel-Gaber and Sohair Ramadan Fahmy

Background and Objective: Sepsis is a universal that represents a physiologic, pathologic and biochemical abnormalities disorder stimulated by infection. The present study aimed to synthesize and apply silver/chitosan/ascorbic acid Nanocomposites (Ag-CS-AA NC) on septic rats induced by cecal ligation and puncture. Materials and Methods: The synthesized Ag-CS-AA NC were characterized by UV-visible spectroscopy, XRD, XPS, FTIR and TEM. H₂O₂ scavenging and antibacterial activities of Ag-CS-AA NC were analyzed. Eighteen rats were randomly divided into three groups: Sham group, CLP-induced sepsis and septic rats treated with Ag-CS-AA NC. Results: The synthesized Ag-CS-AA NC showed in vitro potent antioxidant and antibacterial activities against Gram-positive and Gram-negative bacteria. Ag-CS-AA NC administration to septic rats showed a reduction in alanine aminotransferases, aspartate aminotransferase, alkaline phosphatase, gamma-glutamyltransferase and concentrations of total proteins, albumin, creatinine, urea, uric acid, nitric oxide, while glutathione reduced and catalase increased. The histopathological investigation showed clear improvement in the hepatic and kidney architecture. Conclusion: The data provide the first experimental demonstration that green synthesize Ag-CS-AA NC has a therapeutic effect against sepsis-induced liver and kidney damage through its antibacterial and antioxidant activities.

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