miR-193a-3p Overexpression Inhibits Proliferation and Enhances Paclitaxel Chemosensitivity in Human Non-Small-Cell Lung Cancer Cells

Youli Ke, Xiaowei Yang and Dong Luo

Background and Objective: Non-Small-Cell Lung Cancer Cells (NSCLC) exposes the uppermost transience rates amongst several types of cancer and it is the most recurrent cancer in the globe. This study was purposed to show the potential therapeutic role of miR-193a-3p as a promising tumour suppressor in human A549 NSCLC in combination with paclitaxel. Materials and Methods: A549 cells were treated with miR-193a-3p mimics and paclitaxel, alone and in combination. The cytotoxic effects of microRNA-193a-3p and paclitaxel were determined using an MTT assay. Apoptosis was assessed by ELISA cell death assay. Gene expression analyses were determined by quantitative Reverse transcription-polymerase Chain Reaction (qRT-PCR). Results: Results showed that the combination therapy reduced cell viability with an increase in apoptosis. In addition, miR-193a-3p replacement increased the expression levels of caspase-3 and caspase-9 and decreased the expression levels of MMP-9, vimentin and ROCK in combination treatment compared to the mono treatments groups. Conclusion: The findings suggest that miR-193a-3p replacement combined with paclitaxel could be considered as a new potential therapeutic approach for the improvement of NSCLC treatment.

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