Variation of Resistance and Physiological Response of Orchid from Induction Trichoderma to Infection Odontoglossum ringspot virus (ORSV)

Mahfut , Abdulrasyid Tolangara, Hasna Ahmad, Rusman Rasyid and Zulkifli Ahmad

Background and Objective: The OMF Trichoderma is a biocontrol capable of increasing plant resistance to Odontoglossum ringspot virus (ORSV) infection. The objective of this study was to determine the resistance and physiological responses of orchids induced by OMF and infected with ORSV. Materials and Methods: The study was arranged in a factorial block randomized design. Orchid type (factor 1): Phalaenopsis amabilis (A1) and Dendrobium discolor (A2). Inoculation treatment (factor 2): Control (K), mycorrhiza (M), virus (V) and mycorrhizal-virus (MV). The parameter observation was a response to plant resistance through symptoms of infection, the intensity of disease infection and level of plant resistance, as well as knowing the physiological response of plants through analysis of chlorophyll content. Results: The OMF Trichoderma is proven to be able to prevent the appearance of symptoms of infection and disease development. The results of mycorrhizal and virus inoculations on orchids cause more severe symptoms of infection than those inoculated with viruses only. The OMF Trichoderma also has not been able to increase plant resistance in both orchids. The results of mycorrhizal and viral inoculations on average showed a very susceptible response compared to treatments that were only inoculated with viruses. Total chlorophyll (a, b and total) was induced by OMF and ORSV inoculation on P. amabilis and D. discolor was the same. Conclusion: The results of the analysis of observational data showed the resistance of P. amabilis and D. discolor to infection of ORSV as a result of OMF Trichoderma induced through resistance and physiological responses, there was no difference in resistance between the two. It can be the basis for efforts to protect orchids against disease infections through OMF induction.

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