Evaluation of Chelex 100 for DNA Extraction in Tomato Seedling (Solanum lycopersicum)

Mehrnoush Aminisarteshnizi, Matlawa Mohlabe and Rebotile Lediga

Background and Objective: Tomato (Solanum lycopersicum) is a vegetable crop commonly used as a fresh vegetable or as a spice for food preparation. Extraction of genomic DNA with high quality for this critical vegetable is one of the basic needs of polymerase chain reaction. In modern research, PCR has found wide applications. Materials and Methods: This study had compared two different extraction DNA methods from tomato (S. lycopersicum). The DNA from the tomato was extracted using Chelex "method 1" (overnight incubation at 56°C) and "method 2" (Ten minutes incubation at 95°C) from the fresh leaves of the tomato. For this comparison, we used four samples of S. lycopersicum from South Africa during 2021. Quantitative and qualitative parameters were measured using a spectrophotometer. To confirm and evaluate the extracted DNA, the PCR reaction with primers for 28S was performed on all samples. Results: The results for the spectrophotometer showed that the highest quality of extracted DNA was in "method 2" (1.59-1.64). However, the protein (1.46-1.50 mg mL^–1) was detected in the tested samples through "method 1".The qualitative and quantitative tests for PCR reaction showed that the DNA extracted using "method 2" had better quality than "method 1". Amplification of samples with 28S primer showed higher concentration and purity of DNA extracted with "method 2". Conclusion: In conclusion, both methods worked, but method two showed better results regarding time and high-quality DNA.

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