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Asian Network for Scientific Information is a leading service provider to the publishers of Science, Technology and Medicine (STM) in Asia. Currently Asian Network for Scientific Information is serving more than 37 peer-reviewed journals covering a wide range of academic disciplines to foster communication among scientists, researchers, students and professionals - enabling them to work more efficiently and intelligently, thereby advancing knowledge and learning.

Research Journal of Immunology
eISSN: 2077-2211
pISSN: 1994-7909

Editor-in-Chief:  Gaurisankar Sa
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Research Article
Serum Endoglin and IL-6 Levels as Complementary Diagnostic Biomarkers for Hepatocellular Carcinoma in Egyptian Liver Cirrhosis Patients
M. A. Abdelwahab, M. M. Elbedewy, A. M. Amin and A. K. Tawfik
Background and Objective: Hepatocellular carcinoma is one of the most common fatal diseases in the Egyptian population. Alpha-fetoprotein is widely used for hepatocellular carcinoma screening and diagnosis. Now-a-days, it couldn’t be considered as an effective diagnostic tool due to its low sensitivity and specificity. So, the aim of this prospective study was to evaluate the diagnostic value of serum endoglin and IL-6 as complementary biomarkers in hepatocellular carcinoma patients with underlying cirrhosis compared to alpha-fetoprotein. Materials and Methods: There were 70 individuals included and divided into three main groups, group I, 30 liver cirrhosis patients, group II, 30 liver cirrhosis patients with associated hepatocellular carcinoma and group III, 10 matched healthy volunteers as a control group. Serum IL-6 and endoglin levels were estimated in all subjects using the ELISA technique. Results: In group II patients, the sensitivity of endoglin and IL-6 was 93.3 and 83.3%, respectively versus 60% for α-fetoprotein. The combined use of endoglin and α-fetoprotein improved the sensitivity to 97% while the combined use of IL-6 and α-fetoprotein improved the sensitivity to 94%. Conclusion: Based on the results reported in this study, it was found that endoglin and IL-6 are very useful tools for the diagnosis of hepatocellular carcinoma in Egyptian cirrhotic patients. They were found more specific and sensitive than AFP. Both serum markers showed significant higher levels in hepatocellular carcinoma patients compared to the liver cirrhosis patients and the control group. Endoglin and IL-6 were found to be more sensitive, specific and reliable serum markers than α-fetoprotein for diagnosis and early detection of liver cirrhosis associated hepatocellular carcinoma. The combined use of each one of them with α-fetoprotein can improve the sensitivity and the specificity in the diagnosis of hepatocellular carcinoma. So, they could be useful complementary biomarkers in the diagnosis of liver cirrhosis associated hepatocellular carcinoma.
Research Article
Comparative Studies on the Responses of Some Biochemical Markers in Immuno-Suppressed Rats Treated with Gongronema latifolium and Vernonia amygdalina Ethanol Leaf Extract
Joshua Parker Elijah, Eze Chuma, Ukegbu Chimere Young, Akabike Chigozie Adrain and Igwe Chinagolum Arinzechukwu
Background and Objective: Gongronema latifolium and Vernonia amygdalina are medicinal plants that have been used in the treatment of nausea, hypertension, diabetes, stomach ache, high cholesterol level etc. However this research was set out to access comparatively the immuno-stimulatory effect of ethanol leaf extract of Gongronema latifolium and Vernonia amygdalina in cyclophosphamide immuno-suppressed rats. Materials and Methods: Thirty Wistar albino rats of 5 rats per group were used for this study and were divided into 6 groups. Group 1 rats were normal control; group 2 (positive control) rats were administered cyclophosphamide only, at the dose of 50 mg kg–1 b.wt. i.p. Group 3 rats were administered low dose (400 mg kg–1 b.wt.) of the Vernonia amygdalina leaf extract after cyclophosphamide induction while group 4 rats were administered high dose (600 mg kg–1 b.wt.) of the extract after cyclophosphamide induction. Group 5 rats were administered low dose (400 mg kg–1 b.wt.) of Gongronema latifolium leaf extract after cyclophosphamide induction while group 6 rats were administered high dose (600 mg kg–1 b.wt.) of the extract after cyclophosphamide induction. The results were analyzed using one way analysis of variance (ANOVA) using SPSS version 20. Results: Treatment with Vernonia amygdalina and Gongronema latifolium leaf extract (group 3, 4 and group 5, 6 respectively) showed a significant increase (p<0.05) in SOD activity, glutathione and vitamin C concentrations when compared with group 2 rats treated with cyclophosphamide only. More so treatment with Vernonia amygdalina and Gongronema latifolium leaf extract (group 3, 4 and group 5, 6 respectively) showed a significant decrease (p<0.05) in the liver marker enzymes (ALT, AST and ALP) when compared to group 2 rats treated with cyclophosphamide only, however group 2 rats showed a significant decrease (p<0.05) in total protein concentration and a significant increase (p<0.05) in the total cholesterol concentration and triacylglycerol concentration when compared to the treatment groups. Conclusion: Comparatively, these findings indicate that Vernonia amygdalina may have better antioxidant properties and also exhibited more potency in inhibiting liver damage by lowering the liver marker enzymes compared to Gongronema latifolia.

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