Asian Network for Scientific Information is a Science, Technology and Medicine (STM) publisher of 37 peer-reviewed open access quality journals. We foster communication among scientists, researchers, students and professionals - enabling them to work more efficiently and intelligently, thereby advancing knowledge and learning.

Plant Pathology Journal
eISSN: 1812-5425
pISSN: 1812-5387

Editor-in-Chief:  Mohamed Abdul Rahman Elwakil
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Research Article
Published on March 15, 2016
Hydrogen Peroxide and Acetylsalicylic Acid Induce the Defense of Lupine Against Root Rot Disease
K.M. Abd El-Hai, M.A. El-Metwally and Naglaa T. Mohamed
Isolation of pathogenic fungi from both cultivars of diseased lupine was carried out in five districts of Dakahlia governorate. The high frequency isolated fungi presented in Temi El-Amdeed followed by Bani-Ebeed district. Fusarium solani and F. oxysporum proved to be the most dominate isolated followed by Rhizoctonia solani . In greenhouse, Giza 1 was high susceptible cultivar for infected with root rot pathogenic fungi. Sclerotium rolfsii followed by R. solani then F. solani were the most aggressive damping-off disease. In the field experiment, Giza 2 cultivar was the best in germination% and more tolerant of damping-off than Giza 1. The application of Rhizolex-T50 followed by H2O2 at low concentrate (0.50 mM) showed a highest percentage of germination within lowest percentage of damping-off. With considerable that, no significant differences between Rhizolex-T50 and low concentrate of H2O2. The high photosynthetic pigments and phenolic content were obtained from the application of acetylsalicylic acid (ASA) at moderate concentrate (15 mM) in both cultivars. Giza 2 gave the highest values in these parameters. Soaking both cultivars of lupine seeds in both tested materials increased significantly growth parameter, yield components and seed quality. The moderate concentration of ASA (15 mM) was the most effective followed by the low concentration of H2O2 (0.50 mM). The data suggested that the application of H2O2 at 0.50 mM and ASA at 15 mM as seed soaking could be considered as fungicide alternatives for controlling lupine root rot disease as well as improve growth and productivity.
Research Article
Published on March 15, 2016
Biological Control of Root-rot on Mungbean Plants Incited By Macrophomina phaseolina Through Microbial Antagonists
Shumaila Shahid and Mujeebur Rahman Khan
Present study was carried out to examine the biological potential of different fungal and bacterial antagonists viz., Trichoderma harzianum, T. reesei, Aspergillus niger and Bacillus subtilis in management of root-rot disease caused by Macrophomina phaseolina and on the growth parameters of mungbean under pot condition. From the results, it was evident that the microbial antagonists significantly suppressed the root-rot disease (27-51%) and improved the growth and yield of mungbean. However, the root-rot fungus caused excessive rotting of roots and stunting of plant growth in the untreated plants. Among the tested biological control agents against M. phaseolina, T. harzianum was found to be the most effective against the fungus and resulted in 51% decrease in the root-rot severity and 31% increase in the yield, followed by B. subtilis. Root-rot infection significantly reduced functional root nodules by 58%. Biochemical components viz., total chlorophyll, total carotenoid and leghaemoglobin content was also decreased by 48, 38 and 25%, respectively, in the root-rot fungus infected plants. However, treatment with the biological control agents significantly increased the biochemical components as well as root nodulation in the order T. harzianum>B. subtilis>T. reesei>A. niger. Soil population of M. phaseolina in untreated pots increased exponentially but the increase was 30-66% less in the pots treated with biological control agents. The study has clearly demonstrated that these microbial antagonists specially, T. harzianum and B. subtilis significantly managed the root-rot disease as well as improved the plant growth and yield of mungbean.
Research Article
Published on March 15, 2016
Prevalence and Serological Detection of Apple Viruses in Himachal Pradesh
V.S. Katwal, A. Handa, P.D. Thakur and M. Tomar
Investigations were conducted to record the incidence of viruses in apple orchards of the state along with serological detection by employing ELISA. Seventeen apple orchards surveyed in Shimla, Kinnaur and Kullu district of HP revealed the presence of viral infection with an incidence ranging from 5.41-92.18%. Direct Double Antibody Sandwich (DAS ) ELISA assays resulted in detection of ACLSV, ApMV, ASGV and ASPV in apple trees of different commercial cultivars being grown in different orchards. The ACLSV, ApMV, ASGV and ASPV were found to be present in serologically detectable limit in leaves, bark and petals parts of an infected plant. However, leaf sample drawn during April and May, bark during August and petals taken during April found to contain highest relative concentration of these viruses based on the OD values obtained in serological reactions. About 50% of the diseased plants revealed the presence of two or more than two viruses, thus indicating prevalence of mixed infection.
Research Article
Published on March 15, 2016
Expression of Hydrolytic Enzymes During Interaction of Moniliophthora roreri, Causal Agent of Frosty Pod Rot and Theobroma cacao Pods
C. Torres-Palacios and M. Ramirez-Lepe
Moniliophthora roreri is a hemibiotrophic fungus causal agent of Frosty Pod Rot (FPR) in cacao (Theobroma cacao) pods. The M. roreri causes one of the most economically important diseases of Theobroma cacao in producing areas in Mexico, Central and South America. The hemibiotrophic fungi have genes that allow them to infect pods by penetration through the surfaces of the cacao pod. Until now, it is not yet clear what is the mechanism used by M. roreri to penetrate the cacao pod. In this study the induction of the genes related to this phenomenon was evaluated in order to propose a mechanism of infection. Production of chitinases, chitosanases, glucanases, proteases, lipases and cutinases were induced in vitro by growing M. roreri 111A in 1% (w/v) of colloidal chitin, chitosan, laminarin, casein, tween 20™, apple cutin and cocoa pod shell. Cacao pod shell used as substrate induced the highest enzyme specific activities of chitinases (1.338), chitosanases (0.430), glucanases (0.430) and cutinases (0.167) compared to chitin (1.010), chitosan (0.316), laminarin (0.301) and apple cutin (0.040) in 12 h. The effect of carbon source on enzyme activity in a “Cross-inducted” way was evaluated. Tween 20 induced higher specific activities of chitinases (10.31), chitosanases (9.38), glucanases (7.89), lipases (4.66) and cutinases (5.32). Conversely casein did not increase the proteolytic activity (3.27) as did laminarin (4.45), chitin (4.28) and glucose (3.90). The levels of gene induction obtained by RT-PCR cultured with cocoa pod shell at 12 h showed that a chitinase, a lipase and a cutinase had significant differences (p = 0.05) compared to a basal expression with glucose. The data suggest that these enzymes may be involved in the early stages of the infection process by M. roreri.
Research Article
Published on March 15, 2016
Variable Disease Response to Spot Blotch in Different Eat Varieties and it Assessment at Biochemical and Genetics Level
S.K. Biswas, Mohd. Rajik, Muneeshwar Sharma, Prem Naresh, Upesh Kumar, Kishan Lal and Ravindra Singh
Disease response to spot blotch by different varieties of wheat namely K 307, K 9644, K 9162, K 9351, K 9465 and HD 2985 revealed that all the varieties give variable response on disease severity. Among the varieties, minimum disease severity was recorded in K 9351 with the value of 30.07% at 65 days of plant. Biochemical analysis of leaves of different varieties of wheat revealed that the variable amount of phenol and soluble protein content. The maximum with 31.50 and 28.60 mg g–1 of soluble protein and 3.10 and 2.85 mg g–1 of total phenol content was found in K 9351 at 20 and 65 days of plant, respectively. The correlation co-efficient between disease severity and soluble protein and total phenol content at 65 days of wheat varieties revealed that there was negative correlation showing (r) -0.974 and -0.953. The regression equation of soluble protein and disease severity showed that higher regression value, lower disease incidence. It is also cleared that increase amount of total phenol and protein content both are associated with decreases of disease severity and both the parameter are reduced with the increase with age of plant. The RAPD analysis of different wheat varieties revealed that variable number and size of fragments are found among the varieties. The highest numbers of fragments are found in variety K 9351 which is 13. The presence or absence of fragment and their variable size might be the responsible for morphological and biochemical variability along with resistance response to spot blotch.
Short Communication
Published on March 15, 2016
Molecular Cloning and Sequencing of Telosma Mosaic Virus (TeMV) Causing Mosaic Disease on Patchouli
Rita Noveriza, Gede Suastika, Sri Hendrastuti Hidayat and Keiko T. Natsuaki
Patchouli belonging to the family Lamiaceae is one of the important aromatic plants that originated from the Philippines. Telosma mosaic virus (TeMV) is one of the dominant types of viruses which attack Indonesian patchouli causing mosaic symptoms on the leaves. Only few research publications were conducted in Indonesia, hence this study was conceptualized in the information on the genome structure of the virus was not enough. The nucleotides sequence encoding the Coat Protein (CP) of TeMV-I (Indonesia isolate) was cloned and determined to complete information about this virus and designed an original primers for sequencing of the genes of NIb and NIa of TeMV-I. A part of genome structure of TeMV was sequenced from patchouli plants showing mosaic symptoms by using universal primer from the genus Potyvirus, then cloned to pGEM-T vector. Sequence analysis was done with program Sequence Scanner and CLC Sequence Viewer. The result showed that newly primer set for NIa and NIb region was design and cloned to TeMV-I. It was determined a sequence of 3805 nucleotides (nt) or a polypeptide chain of 1193 amino acid (aa) at the 3’-terminal region of the genome of TeMV. This contained the 3’-terminal part of the NIa (nts 1-1182), the NIb (nts 1183-2733), the coat protein (nts 2734-3549) and the 3’-UTR (nts 3550-3805). The stop codon (TAA) was followed by a 3’-untranslated region (3’-UTR) of 253 nt. The sequence analysis and development of specific primers for TeMV will be of great help in the detection and protection of this virus.

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