Asian Network for Scientific Information is a Science, Technology and Medicine (STM) publisher of 37 peer-reviewed open access quality journals. We foster communication among scientists, researchers, students and professionals - enabling them to work more efficiently and intelligently, thereby advancing knowledge and learning.

Plant Pathology Journal
eISSN: 1812-5425
pISSN: 1812-5387

Editor-in-Chief:  Mohamed Abdul Rahman Elwakil
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Research Article
Published on June 27, 2015
ATMPK4 and ATMPK6 Transcript and Protein Profiling in Arabidopsis thaliana Plants Challenged with Zeatin and Alternaria brassicae
A.K. Gupta, M. Baunthiyal, D. Jeena and D. Pandey
Mitogen-Activated Protein (MAP) kinases play very important role in signal transduction during pathogen attack. We attempted to study the response of MAPK4 and MAPK6 under three different conditions i.e., with pathogen (Alternaria brassicae) only, with zeatin only and with both pathogen and zeatin. The study was performed on Arabidopsis thaliana (A. thaliana) plants. The objective was to identify the relationship between zeatin and disease resistance. To our surprise, we found an increased disease incidence in presence of pathogen at middle stage along with zeatin and also observed increased expression of MAPK4 under similar condition. MAPK6 expressions are already known to be increased during the disease incidence and in our case it has increased expression during the application of both pathogen as well as zeatin. Two-dimensional (2D) analysis of these samples revealed that the upregulated proteins were mostly in the range of 35-50 kDa range which is similar to the range of where all the MAP kinases.
Research Article
Published on June 27, 2015
Pathogenic Fitness, Metalaxyl Sensitivity and Isozyme Analysis of Phytophthora infestans Isolates from Mauritius
A. Ibrahim and N. Taleb-Hossenkhan
Phytophthora infestans, the causal agent of potato and tomato late blight disease continues to be a serious threat in almost all potato and tomato growing areas around the world, with dramatic and disastrous economic consequences. In Mauritius, the disease is reported every year and many of the commercial potato varieties (Spunta, Delaware and Mondial) previously found to be moderately resistant or susceptible to the disease, have now become highly susceptible. This study reports the first phenotypic and genotypic characterization of local strains of Phytophthora infestans from the island of Mauritius. Seven isolates of Phytophthora infestans were collected from distinct geographical areas during the blight seasons of 2012 and 2013 and tested for aggressiveness on four potato and three tomato cultivars, metalaxyl sensitivity and subjected to isozyme analysis at the Gpi and Pep loci. Results show that all the isolates exhibited a clear host adaptation to potato, being only very weakly pathogenic on tomato. Most of the isolates (five out of seven) had high or intermediate metalaxyl resistance. Isozyme analysis revealed that at the Gpi locus, two isolates were 86/86, one isolate was 86/100, one isolate was 100/100 and one isolate was 122/122. At the Pep locus, two isolates were 92/92, two isolates were 92/100 and three isolates were 100/100. The combined results of the tests indicate that the seven isolates collected over the island are genetically distinct from each other and do not represent the same strain.
Research Article
Published on June 27, 2015
Sequence Motif Comparison and Homology Modeling of Helper Component Proteinase (HC-Pro) of Banana bract mosaic virus
Selvarajan Ramasamy, Balasubramanian Velusamy and Anuradha Chelliah
The Helper Component Proteinase (HC-Pro) is a key protein encoded by the plant viruses of the genus potyvirus. The HC-Pro is involved in different steps of the viral cycle, aphid transmission, replication virus cell-to-cell and systemic movement and is a suppressor of post-transcriptional gene silencing. The amino acid sequence of Banana bract mosaic virus (BBrMV) HC-Pro was compared both inter and intra species across different potyviruses to demonstrate the functional correlation of sequence motifs of HC-Pro to predict its role in virus cycles. Knowledge on the structure of HC-Pro will greatly contribute to understand its multifaceted functions and how structural domains are organized to fulfill them in concert or independently hence, a stable model of BBrMV HC-Pro has been proposed based on homology modeling. The results obtained from this study will provide a framework for new hypothesis and research directions in the area of differential role of HC-Pro.
Research Article
Published on June 27, 2015
Use of Chitosan to Control Crown Gall Disease
Hanan A. Shaheen, A.F. Abd El- Rahman, H. Abd El- Ghany and Z. Moussa
The antibacterial activity of chitosan solution against Agrobacterium tumifaciens was investigated in this study. The in vitro antibacterial effect of chitosan against A. tumifaciens was affected by chitosan concentrations, pH value, concentration of acetic acid used to dissolve chitosan and incubation time. Chitosan concentrations 2.5 mg mL–1 and 5 mg mL–1 exhibited strong antibacterial activity at the pH 6.6 and 5.6, respectively. The gall diameter and gall weight of tomato seedlings at dipping time 10 and 20 min was significantly reduced by chitosan concentrations 2.5 and 5 mg mL–1 at pH 6.6 and 5.6, respectively. The viable bacterial counts after 8 h of incubation in chitosan concentration 5 mg mL–1 at pH 5.6 was 0.0 log CFU mL–1.
Research Article
Published on June 27, 2015
Association of Mycoflora with Coffee (Coffea arabica L.) Beans at Limmu Coffee Plantation, Southwestern Ethiopia
Weyessa Garedew, Girma Adunga, Amsalu Abera and Fikre Lemessa
Coffee (Coffea arabica L.) bean quality can be affected by a number of factors of which storage fungi are one of the major ones. In Ethiopia coffee is a number one export commodity supporting the national economy but there was little information about the association of mycoflora with coffee beans. Therefore, the objective of this study was to assess the associations of mycoflora with coffee seeds and their effect on coffee infection at Limmu Coffee Plantation. The comparison was done using two coffee berry disease resistant selections (74112 and 74110) with and without parchment and with and without surface treatment of coffee beans with 5% sodium hypochlorite and storing under two storage conditions (local cold house and corrugated iron warehouse). Thus, the experiment was laid down as 2×2×2×2 factorial experiment with four replications. The study showed the association of four fungal species (Fusarium spp., Aspergillus spp., Penicillium spp. and Mucor spp.) and some unidentified species in both blotting and agar plating techniques. In general, significantly higher infection percentage was found in coffee seeds without parchment and surface disinfection irrespective of the storage type and coffee selection. Thus, keeping coffee beans with parchment and disinfecting by disinfectants may reduce the association and prevalence of mycoflora on coffee and minimize postharvest problems.
Research Article
Published on June 27, 2015
Isolation and Molecular Characterization of the Fire Blight Pathogen, Erwinia amylovora, Isolated from Apple and Pear Orchards in Egypt
Moustafa A. El-Sabagh, Taha I. Zaghloul and Nader A. Ashmawy
Eleven Erwinia amylovora isolates, the causal agent of the fire blight disease, were isolated from apple orchards adjacent to those of pears showing fire blight disease symptoms. Initial characterization concerning morphological and biochemical traits revealed that all isolates were typical to those of E. amylovora. Furthermore, all bacterial isolates showed typical symptoms of the fire blight disease upon infection of unripe pear fruits. Molecular characterization of these isolates via PCR utilizing specific primers based on a region of plasmid pEA29 and chromosomal DNA ams–region was performed. PCR products were positive and represented the expected length 1.1 and 1.6 kb, respectively. Moreover, PCR reactions utilizing the 16S rRNA universal primers were carried out. DNA sequence of PCR products and analysis via blast and Genbank data showed that, the bacterial isolates actually belonging to E. amylovora. Moreover, there are interference between these Erwinia isolates and identified E. amylovora strains based on constructed phylogenetic analysis. To the best of our knowledge this is the first report of fire blight of apples caused by E. amylovora in Egypt.
Research Article
Published on June 27, 2015
Effects of Tannin to Control Leaf Blight Disease on Toona sureni Merr. Caused by Two Isolates of Rhizoctonia sp.
Bonny P.W. Soekarno, Muhammad Alam Firmansyah, Achmad and Arief B. Witarto
This study investigated the efficacy of tannin in order to control leaf blight disease on Toona sureni caused by Rhizoctonia sp. by measuring disease intensity and percentage of leaf damages. Two Rhizoctonia sp. (R1 and R2) isolates were induced on the T. sureni leaves. Tannin solution with concentration of 0.5, 1, 2 and 3% and control (water) were sprayed on the leaves surface every two days. The percentage of disease sign on the leaves was measured every two days for one month. Results revealed that all tannin solution treatments reduced intensity and leaf death percentage. No poisonous impacts on the seedling were observed. The highest tannin concentrations which reduce disease activity were 3% for both isolates. It reduced the disease by stopping the fungal enzymatic activity of cellulolytic and pectinolytic. The highest increase of disease intensity and the percentage of leaf death was found in the control treatment. It can be concluded that tannin could be used to control leaf blight disease on Toona sureni caused by Rhizoctonia sp.
Research Article
Published on June 27, 2015
Efficacy Evaluation of Iraqi Propolis Against Gray Mold of Stored Orange Caused by Penicillium digitatum
Oadi N. Matny
This study was conducted to evaluate Iraqi propolis against gray mold on orange. Propolis Ethanolic Extract (PEE) in three concentrations 1, 2 and 3% were used to treated orange treatments separately and storage at 25±2°C for three weeks. Results showed a significant reduced in disease severity in the first, second and third week for all PEE concentrations compared with infected control. The best treatment was 3% in disease severity reduction. Disease incidence was significantly reduced with the treatment treated with PEE for all concentrations compared with the pathogen treatment, both concentration of PEE 2 and 3% was the most effective. Also, all PEE concentrations reduce depth of the mold area compared with pathogen treatment. There are no significant differences in patulin production between PEE and pathogen treatments was founded.

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