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International Journal of Botany
eISSN: 1811-9719
pISSN: 1811-9700

Editor-in-Chief:  Abdelfattah Badr Mohamed Badr
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Research Article
Using Ringing Branches Technique and Natural Lipids to Enhance Quality and Storability of Florida Prince Peach Fruits During Cold Storage
Mohamed Tarabih and Eman EL-Eryan
Background: In Egypt, Florida prince peach an early harvest cultivar that adapt to local environmental conditions and potential for exportation. Color is variable as the global maturity indicator in peaches. Materials and Methods: In this respect, influence of ringing main branches technique at the beginning of coloring (4 weeks before harvest) with pre-harvest application of lysophosphatidylethanolamine (LPE) 2 weeks before harvest on quality and storability of Florida prince peach fruits were investigated during two successive seasons 2016 and 2017. The fruits were stored 30 days at 0°C±1 with 90-95% RH and fruit quality was evaluated at harvest and during cold storage. Results: At harvest, all treatments were more effective to increase fruit firmness, total soluble solids, total sugars content, color hue angle (h°) compared to the control in both seasons. While, produced a lower total acidity, total phenols, the activity of peroxidase and polyphenol oxidase. Results showed that by increasing the storage periods weight loss, decay, total soluble solids and total sugars content, the activity of peroxidase and polyphenol oxidase significantly increased. Whereas, fruit firmness, total acidity, color hue angle (h°) and total phenols significantly decreased. The best results obtained by sprayed LPE at 150 mg L–1 with ringing main branches after 30 days of cold storage which decreased fruits weight loss, decay, delayed the changes in total acidity, total phenols, activity of peroxidase and activity of polyphenol oxidase. Moreover, the same treatment produced a higher value of total soluble solids, total sugars and color hue angle (h°) compared with all treated fruits or the untreated ones. Conclusion: The study suggested that these treatments might be a promising candidate as to maintain peach quality and extending post-harvest life especially after cold storage.
Research Article
Genetic Fidelity Testing of Soybean Plants under Allelopathic Stress of Eucalyptus Ground Leaves Through RAPD and ISSR Markers
Hala Mohamed Abdelmigid and Maissa Mohamed Morsi
Background and Objectives: In agroforestry system (e.g., Eucalyptus forestry), plant-plant chemical interactions can have a strong impact on the biodiversity and dynamics of these ecosystems. The objective of the study was to evaluate the genetic stability of crop plants under allelopathic stress of tree litter. PCR based analysis system (RAPD and ISSR) was employed to determine molecular markers associated with allelopathic tolerance in Glycine max. Materials and Methods: Pot experiment was conducted with soybean seeds using mixture of soil and Eucalyptus ground leaves (EUGL) in a percentage of 0 (control), 10, 20, 30, 40, 50, (w/w). Leaves were harvested after 3 weeks for DNA extraction and further PCR assays. Genetic fidelity testing was assessed by calculations of genome template stability index (GTS). Results: Collectively, 18 new DNA markers were detected by both RAPD and ISSR analysis, in EUGL exposed plants. The RAPD and ISSR profiles verified a general tendency of decrease in GTS values with exposure to EUGL in a dose-dependent manner. A drastic decrease in GTS was more pronounced by RAPD markers, compared to ISSR. Low estimated GTS values for EUGL plants reflect their high genetic instability. Conclusion: The RAPD seven markers and ISSR 11markers was proved as reliable molecular markers for allelopathy tolerance in Glycine max and could be used in breeding programs. Moreover, lowest estimated GTS values for EUGL plants reflect their high genetic instability.
Research Article
Antioxidant, Antimicrobial Activities and Phytochemical Constituents of Cichorium intybus L. Aerial Parts
Riyam Sabeeh Jasim
Background and Objective: Cichorium intybus Linn. (C. intybus L.) has been known in traditional medicine since ancient times, therefore, the present study aimed to investigate the phytochemical composition, antioxidant activity and antimicrobial potentiality of aqueous and methanol extracts of aerial parts of C. intybus L. Materials and Methods: Total phenolic, flavonoids, alkaloids, tannins and saponins were assessed as the Mean±SD of three readings in the extracts of the aerial parts of C. intybus L. The extracts were examined for their antioxidant and antimicrobial activities. Results: The methanol extract was found to possess a comparatively higher values of total phenolic, flavonoids, alkaloids and tannins than aqueous extract except saponins which seem higher in aqueous than methanol extract. The methanol extract exhibited a relatively high antioxidant activity as it showed comparatively low values of IC50 for 2,2-Diphenyl-1-picrylhydrazyl (DPPH) inhibition than ascorbic acid (standard) and the aqueous extract. Both extracts were screened for their antimicrobial activity against 11 pathogenic microbial strains and showed significant inhibition zones. The methanol extract showed broader antimicrobial spectrum than the aqueous extract. Conclusion: The results of this study indicated that the aerial parts extracts of C. intybus L. have good phytochemical composition, good antioxidant activity and antimicrobial potentiality.

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