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Asian Network for Scientific Information is a leading service provider to the publishers of Science, Technology and Medicine (STM) in Asia. Currently Asian Network for Scientific Information is serving more than 37 peer-reviewed journals covering a wide range of academic disciplines to foster communication among scientists, researchers, students and professionals - enabling them to work more efficiently and intelligently, thereby advancing knowledge and learning.

Asian Journal of Plant Sciences
eISSN: 1812-5697
pISSN: 1682-3974

Editor-in-Chief:  Rajarshi Kumar Gaur
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Research Article
Published on July 27, 2019
Effects of Arbuscular Mycorrhizal Fungi and Organic Material on Growth and Nutrient Uptake by Pericopsis mooniana in Coal Mine
Husna , Irdika Mansur, Sri Wilarso Budi R., Faisal Danu Tuheteru, Asrianti Arif, Edy Jamal Tuheteru and Albasri
Background and Objective: Post-coal mining land has poor soil physical characteristic and high metal toxicity and hence needs to be recovered. The recovery effort can be done through addition of organic material input, utilization of beneficial microorganism such as Arbuscular Mycorrhizal Fungi (AMF) and selection of suitable tree species. The purpose of this study was to investigate the effect of AMF and compost on the growth and uptake of nutrients and metal by Pericopsis mooniana in soil media of post coal mine land. Materials and Methods: A greenhouse experiment was carried out by using factorial Completely Randomised Design. The first factor was the AMF inoculum (Factor A): control (A0), AMF-KG (A1), AMF-UHO (A2), AMF-Vale (A3), AMF-CA (A4), AMF-HA (A5), AMF-BJ (A6), AMF-Mycofer (A7) and the second factor was an organic material (Factor B): ex-mined soil+without any organic material (B0), soil, compost, sand (2:1:1) (B1) and soil, compost, sand and rice husk (2:1:1:1) (B2). The experiment was carried out for 5 months on a greenhouse scale. After 150 days the seedlings were collected to quantify growth parameters, mycorrhizal root colonization rate and number spores and nutrient uptake. Results: The results showed that the local AMF was able to colonize the roots of the P. mooniana and increase in the growth and dry weight of plants and also tend to increase the C, N, P, K, Ca and Mg accumulation. The enrichment of organic material in the coal mine media stimulated the growth and nutrient uptake. P. mooniana performs phytoremediation mechanisms through rhizofiltration techniques (Fe, Cd, Pb) and phytoextraction (Cr and Mn). Conclusion: Inoculation with AMF and the enrichment of organic materials have the potential to support post-coal mining land restoration.
Research Article
Published on July 27, 2019
Effect of Pruning and Cytokinin Application on the Growth of Tea GMB 7 Clone
Intan Ratna Dewi Anjarsari, Jajang Sauman Hamdani, Cucu Suherman and Tati Nurmala
Background and Objectives: The use of growth regulators in pruned tea plantation in Indonesia is still very rare. One method that can be applied to increase growth is through the application of cytokinin such as Benzil amino purine (BAP). The objective of this study was to determine whether the combination of pruning and application of cytokinin BAP can increase the growth of tea. Materials and Methods: This experiment was conducted in Tea and Chinchona Research Centre, Gambung, west Java with an altitude of 1,250 masl. Randomized block design (RBD) was used with pruned height treatment, pruning type and cytokinin dosage combined into 8 treatments. Materials were used 7 year old GMB 7 clone and BAP. All parameters were tested by analysis of variance (ANOVA), followed by Duncan's multiple range test (DMRT) at the 5% significance level. Results: The results showed that type and height of pruning and application of cytokinin BAP affect the growth of pruned tea plants. The combination of clean pruning treatment, a pruning height of 60 cm and cytokinin usage in 60 mL produced optimal results for increased number of buds and active shoot (pecco) in pruned tea plants. Conclusion: The application of BAP can improve plant growth and development. This indicates that the provision of growth regulators has the potential to be used to accelerate the growth of tea plants after being pruned.
Research Article
Published on July 29, 2019
Physiological Characteristics of Binahong (Anredera cordifolia (Ten.) Steenis) on Application of Natural Plant Growth Regulator
Yaya Hasanah, Lisa Mawarni and Herla Rusmarilin
Background and Objective: Natural Plant Growth Regulator (PGR) not only affects the growth and yield of binahong, but also affects the physiological characteristics of binahong. The objective of the research was to determine the impact of the foliar application of natural PGR (shallot extract, banana hump extract and bamboo shoot extract) on physiological characteristics of binahong. Materials and Methods: The research use a non-factorial randomized block design with 7 treatment and 4 replication. The natural PGR treatment in the research was without natural PGR ; shallot extract 40 mL nL1; bamboo shoots extract 40 mL L1; banana hump extract 40 mL L1; shallot extract (20 mL L1)+bamboo shoot extract (20 mL L1); shallot extract (20 mL L1)+banana hump extract (20 mL L1); bamboo shoots extract (20 mL L1)+banana hump extract (20 mL L1). The variables observed were chlorophyll a, chlorophyll b, total chlorophyll, density of stomata and cuticle thickness. Data were analyzed by using analysis of variance at p = 0.05. Results: The result showed that the application of natural PGR decreased stomatal density compared to control (without natural PGR application), but increased chlorophyll a, chlorophyll b and total chlorophyll. Natural PGR of shallot extract 40 mL L1 application increased total chlorophyll. Banana hump extract 40 mL L1 application increased the cuticle thickness compared to other natural PGR. Conclusion: It was concluded that application of natural PGR decreased the stomatal density, but increased the total chlorophyll. Banana hump extract 40 mL L1 application increased the cuticle thickness compared to other natural PGR.
Research Article
Published on July 29, 2019
Growth Response and Production of Purple Sweet Potatoes after Provision of Arbuscular Mycorrhizal Fungi and Organic Fertilizer
Johanis Jullian Pelealu, Lalu Wahyudi and Trina Ekawati Tallei
Background and Objective: Indonesia is rich in non-rice carbohydrate sources including tubers and seeds, but the achievement of consumption these kind of food has only reached 5%. One of these tubers is purple sweet potato. This study aimed to examine the effect of administration of Arbuscular Mycorrhizal Fungi (AMF) and Organic Fertilizer (OF) on the growth and production of purple sweet potatoes. Materials and Methods: Purple sweet potato seeds were sown in the form of plant stems. This study used factorial Randomized Block Design (RBD) consisting of 2 factors with 4 replications. The first factor is the dose of AMF and the second factor is OF. The agronomical parameters observed included stem length, number of leaves, number of branches, number of flowers as well as weight, diameter, number and length of tuber. All variables were analyzed with one-way analysis of variance (ANOVA). Results: The results showed that the administration of 20 and 30 g of AMF supplemented with 50 g of OF (m2p1 and m3p1, respectively) significantly increased stem length, number of leaves, number of branches, tuber weight, diameter and length. Statistical analysis showed a highly significant increase in potato yield for inoculated plants compared with non-inoculated controls. Conclusion: Hence, it can be concluded that application of Arbuscular Mycorrhizal Fungi in combination with organic fertilizer were able to improve the plant growth and the tuber yield of purple sweet potato.
Research Article
Published on September 04, 2019
Mass Propagation of Urena lobata L. Hairy Root Possessing α-Glucosidase Inhibitory Activity by Using Suitable Culture Conditions
Vu Thi Bach Phuong, Cao Minh Dai, Bui Lan Anh, Pham Thi Anh Hong and Quach Ngo Diem Phuong
Background and Objective: Urena lobata L., a member of the Malvaceae family, is commonly used as a traditional medicine in tropical and subtropical regions. The leaf and root extracts of Urena lobata L. have been used to treat several diseases such as diabetes, rheumatism, gonorrhea and toothache. This study aims at improving culture conditions of Urena lobata L. hairy root for biomass and α-glucosidase inhibitory activity. Materials and Methods: Urena lobata L. hairy roots were induced by infecting the in vitro leaf explants with Agrobacterium rhizogenes ATCC 15834 and were grown in WPM liquid medium under darkness. Several factors affecting the growth of Urena lobata L. hairy roots including of inoculum sizes (0.05, 0.1, 0.15 and 0.2 g/30 mL FW), concentrations of sucrose (2, 3, 4 and 5%) and macroelements composition (half, full, double and triple-strength WPM macroelements) were investigated. The combitation of improved factors were compared with the original process for biomass and the inhibition of α-glucosidase. Results: The combitation of improved factors using WPM medium with double-strength of macroelements composition supplemented with 4% (w/v) sucrose, an inoculum size of 0.1 g/30 mL FW has higher biomass of hairy root (1.33 times) than the original process. In addition, the hairy root cultivated in combination culture has maintained α-glucosidase inhibitory activity (during 40 days) longer than in individually improved factors and original process. Conclusion: A protocol for Urena lobata L. hairy root culture was established which could produce a material have longer α-glucosidase inhibitory activity.
Research Article
Published on September 23, 2019
Drinking Straw from Coconut Leaf: A Study of its Epicuticular Wax Content and Phenol Extrusion Properties
Jyoti Jeena James, Dylan D. Silva, Saji Varghese, Jobi Xavier and Kuppusamy Alagesan Paari
Background and Objectives: Plastics are a ubiquitous part of our daily life but now posing a major threat to marine life, animal and human health. More than 50% of the manufactured plastic including straws are being disposed of after single-use. There is an increasing need to mitigate this trend so that the damage could be brought under control. The aim of this research was to develop a compostable, eco-friendly alternative to plastic straws using the leaves of Cocos nucifera L. Materials and Methods: The biochemical properties of 6 varieties of Cocos nucifera L. leaflets were studied in order to screen the most suitable material for making sustainable straws. Epicuticular wax content was analyzed to choose the best variety for preparation of hydrophobic straws. Total antioxidant activity, total tannin content, phenolic and flavonoid content were assayed to evaluate the potential functionality of the leaflets. The phenol extrusion properties of the material were also checked in acidic and normal beverages. Results: Estimation of epicuticular wax and phytochemical analysis in all 6 varieties revealed that all varieties of Cocos nucifera L. leaves provide a potent biomaterial for straw preparation. Silicon 732 was found to be a good adhesive agent for straw preparation. Phenol extrusion assays revealed that there is a negligible difference in the release of phytochemicals before and after dipping of straws in the beverages. Conclusion: The outcome of this research opens up vistas to carry out further research in a hitherto unexplored area of utilizing the leaf of Cocos nucifera in a novel way with far reaching economic and employment implications.

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