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Asian Journal of Plant Sciences
eISSN: 1812-5697
pISSN: 1682-3974

Editor-in-Chief:  Sayyada Khatoon
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Research Article
Effect of Nitrogen and Phosphorus on the Growth and Redox Homeostasis of Salt-stressed Mustard Plants
Naveed Gulzar, Mohammad Yaseen Mir and Saima Hamid
Background and Objective: Salt stress brings about a considerable change in the physiological and biochemical processes which significantly hampers the growth and productivity of the plants to a greater extent. Materials and Methods: Indian mustard was chosen as plant material. The seeds were washed thoroughly with water and surface sterilized with 0.01% mercuric chloride and washed again with distilled water prior to sowing in the pots containing a mixture of sand and vermiculite (1:1). Four different treatments were given to 20 days-old plants viz. NaCl (T1), NaCl+N (T2), NaCl+P (T3) and NaCl+N+P (T4). Standard methodology was utilized to carry out the research. Results: In the present study four experimental lines were set up simultaneously viz. NaCl (T1), NaCl+N (T2), NaCl+P (T3) and NaCl+N+P (T4). The treatments include 50 mM Nacl, 100 mM Nitrogen and 100 mM Phosphorus. The T4 samples showed significant results with respect to NaCl stressed plants. Conclusion: Thus our results suggest that overall growth and productivity of the salt stressed plants can be improved by the combined application of nitrogen and phosphorus fertilizers.
Research Article
Improvement of Blast Resistance and Yield Productivity in Rice (Oryza sativa L.) Using Tissue Culture and Combining Ability
Mohammed I. Al-Daej, Adel A. Rezk and Mohamed M. El-Malky
Background and Objective: Blast disease is one of the major pathogens affecting crop productivity. Breeding for resistant is economic way to produce resistant variety and tissue culture used as a tool to decrease the period of breeding program. Materials and Methods: Seven varieties (Al-Ahsa Type1, Al-Ahsa Type2, Sakha104, Gz6903, Giza178, Giza177 and Sakha105) were used for tissue cultural and crossing through line X tester method. Somaclonal variation and 12 F1 were evaluated and estimated the GCA and SCA under field condition. The MS with 2,4-D (2,4-Dichlorophenoxyacetic acid) 3% sucrose were used for callus induction and plant regeneration. Results: For callus induction, the varieties Sakha104, Gz6903, Giza177 and Sakha105 gave 100%, while, varieties Al-Ahsa Type1, Al-Ahsa Type2 gave 85%. In addition, 18 lines produced from Al-Ahsa Type1 results showed that 13 lines were resistant to blast and 5 lines were susceptible. In SC1 family derived from Al-Ahsa Type2 results indicated that ten lines were resistant to leaf blast, while 6 lines were susceptible. As for SC1 family derived from Sakha104, 11 lines were produced and results showed that 6 lines were susceptible to blast and five lines were resistant to leaf blast. In field experiment, 12 F1s were produced through Line×Tester and evaluated with their parents for genotypic variation. The results showed that both general combining ability (GCA) variances were highly significant for all characters studied in F1 generations. Conclusion: Tissue culture in traditional plant breeding is one of the most effective means for production new varieties of rice resistant to blast disease as well as improving crop traits. The most promising hybrid combinations were Al-Ahsa Type1×Sakha105, Al-Ahsa Type2×Giza178, Al-Ahsa Type2×Sakha105, Sakha104×Giza177 and Gz6903-1-2-2×Sakha105 for desirable traits and could be utilized in rice breeding program to improve these traits.
Research Article
Impact of Habitat Heterogeneity on Growth Dynamics and Physiological Responses of Dipterygium glaucum
Neelam Sherwani and Sardar Abulfazal Farooq
Background and Objective: Dipterygium glaucum, a rangeland plant holds high ecological importance in desert conservation by providing a vegetation cover along with other species. This study investigated the impact of biotic and abiotic factors across different habitats, resulting in intraspecific variations in different ecotypes of Dipterygium glaucum. Materials and Methods: Morphological variations in different habitats were assessed using 2 way MANOVA and Principal Component Analysis (PCA) with SPSS 23.0 statistical software. Total phenolics were estimated using Folin and Ciocalteu reagent, chlorophyll content was determined using spectrophotometer method. Antimicrobial activity of the plant extract was assessed against 2 g positive and 2 g negative pathogenic bacterial strains. Results: Variations among populations of three habitats due to adaption to varied environmental conditions were significant. The highland variant showed robust growth compared to coastal and sand dune populations and produced 2 types of flowers white and yellow compared to only yellow flowers in other habitats. Decreased chlorophyll content and increased antimicrobial activity and total phenolics were observed under salt stress. Conclusion: Dipterygium glaucum exhibit phenotypic plasticity across different habitats resulting in intraspecific variations and exist as ecotypes.
Research Article
Shoots Culture of Gynura procumbens (Lour.) Merr. in Balloon-Type Bubble-bioreactor Influenced by Sucrose Concentration and Inoculums Density
Ira Nailas Saadah, Alfinda Novi Kristanti, Popy Hartatie Hardjo and Yosephine Sri Wulan Manuhara
Background and objectives: Gynura procumbens has been widely used as a traditional medicine in Indonesia. Micropropagation of this plant was important to fulfill the need of pharmaceutical industry. The aims of this study were to increasing the number of G. procumbens shoots in balloon-type bubble bioreactor influenced by sucrose concentration and inoculums density. Materials and Methods: Nodal segments of G. procumbens were used as explants which were grown in balloon type bubble bioreactor in MS liquid medium supplemented with 2 mg L1 IAA and 4 mg L1 BAP. Shoot culture were conducted under treatment of various concentration of sucrose (10, 30 and 50 g L1) and various inoculums density (5, 10 and 15 explants). Results: The highest multiplication of shoots and growth index were obtained at 50 g L1 sucrose treatment and 5 explants as initial inoculums; there are 9.36 shoots/explants and 13.8 shoots/explants, respectively. Conclusion: The study found that G. procumbens shoots cultured in balloon-type bubble bioreactors increased shoots multiplication significantly.

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