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Asian Journal of Plant Sciences
eISSN: 1812-5697
pISSN: 1682-3974

Editor-in-Chief:  Sayyada Khatoon
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Research Article
Effect of NaCl on Growth and Development of in vitro Plants of Date Palm (Phoenix dactylifera L.) ‘Khainazi’ Cultivar
Saleh Alturki
Background and Objectives: High salinity of soil and water resources is a global issue which is causing substantial decline of productive lands in many arid and semiarid regions of the world due to salinization. Therefore, the main objective of this study was to determine the effect of NaCl on growth and development of in vitro plants of date palm (Phoenix dactylifera L.) ‘Khainazi’ cultivar. Materials and Methods: The study was carried out in the Tissue Culture Laboratory, Date Palm Research Center, King Faisal University, Kingdom of Saudi Arabia. Ten date palm offshoots of cv. Khainazi, about 3-4 years old and weighing 5-7 kg were used in the experiment. The shoot tip and lateral buds were sterilized in 20% v/v clorex solution for 15 min and then sectioned into 1 cm explants for culture initiation. The modified MS media was supplemented with different concentrations of NaCl ranging from 0, 50, 100, 200, 300 and 400 mmol L–1 and incubated at 25±2°C for 16 h in light daily supplied by 65/80 Warm White Weisse 3500 fluorescent tubes. Each treatment was replicated 10 times by following a completely randomized design. Plant observations included dry weight of shoots and roots and length of stem and roots. The concentration of Na, Mg, Ca and K in plant roots and shoots were determined by the Atomic Absorption Spectrophotometer while Cl by a Chloride meter. Experimental data were analyzed by following appropriate statistical procedures. Results: Stem and root length and dry weight were progressively reduced in most of the treatments by the addition of 0, 50, 100, 200, 300 and 400 mmol L–1 to the growth medium. The reduction in shoot, however, was greater than root. The concentration of Na increased significantly both in the shoots and roots. While the concentration of Ca, Mg and K increased with the addition of NaCl up to 100 mmol L–1, then decreased with increasing the NaCl concentration up to 400 mmol L–1 in the growth medium.. Conclusion: Addition of high NaCl concentration to growth medium for the development of date palm off-shoots using tissue culture technology did not show encouraging results. In conclusion, addition of NaCl solution having concentration above 100 mmol L–1 to growth medium proved detrimental to explants to obtain desired date palm off shoots.
Research Article
Characteristic of Vascular Bundles and Morphology of Gigantochloa apus (J.A. and J.H. Schulltes) Kurz Culm
Atmawi Darwis, Ihak Sumardi, Yoyo Suhaya and Sopandi Sunarya
Background and Objective: Bamboo culm was composed of internodes and nodes. Anatomically, bamboo culm was composed of vascular bundles (VB) and parenchymal base tissue. The purpose of this study was to determine the morphology features of culm and VB of bamboo Gigantochloa apus. Materials and Methods: The sample was taken from each internode of bamboo. The morphology of bamboo culm was determined by measuring the length, diameter and wall thickness. VB characterization was obtained through observation on each internode of bamboo culm. Results: The results showed that bamboo culm had internode length and inner diameter that varied from the bottom, increased at a certain height then decreased toward the top. The size of outer diameter and wall thickness of bamboo culm tended to decrease from bottom to top. VB on each internode was spread unevenly where the distance between VB looked more widened from the outer layer to the inner layer. VB distribution in the cross section had a crossing pattern (alternate). VB varied in shape and size from outer layer to inner layer of bamboo culm. The VB type on an internode of G. apus bamboo culm also showed variations where there was VB in type III only and some also were in type (III and IV) in each internode. Conclusion: The morphology of the bamboo culm was influenced by the morphology of young shoot. VB of bamboo G. apus spread unevenly with varying sizes, shapes and type from the outer layer to the inner layer.

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