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Asian Network for Scientific Information is a Science, Technology and Medicine (STM) publisher of 37 peer-reviewed open access quality journals. We foster communication among scientists, researchers, students and professionals - enabling them to work more efficiently and intelligently, thereby advancing knowledge and learning.

Biotechnology
eISSN: 1682-2978
pISSN: 1682-296x

Editor-in-Chief:  Akhtar Jamal Khan
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Research Article
Published on October 15, 2016
Enhancing of Drought-tolerant Rice (Oryza sativa) Variety MRQ74 Through Gamma Radiation and in vitro Pathway
Che Radziah Che Mohd Zain, Ahsan A. Kadhimi, Arshad Naji Alhasnawi, Anizan Isahak, Azhar Mohamad, Febri Doni and Wan Mohtar Wan Yusoff
Background and Objective: Aromatic rice MRQ74, known as Maswangi is an important rice variety in Malaysia. However, drought tolerance is essential for the growth of MRQ74. This study was conducted by exposing naked seeds to acute gamma radiation to induce callus embryogenesis. Methodology: A dose response experiment was performed at the Nuclear Malaysia Agency to expose the naked MRQ74 seeds to 350 Gy; these seeds were cultured to produce callus on Murashige and Skoog (MS) media supplemented with 2,4-D, (1.0, 2.0 or 3.0 mg L–1) and Kin (0.1 and 0.2 mg L–1) for 4 weeks. Under the optimized conditions in media and regeneration, the callus was introduced to drought stress with 1.5, 3, 4.5, 6 and 7% PEG (MW 6000). Results: The most efficient response to callusing was found at 3 mg L–1 2,4-D and 0.1 mg L–1 kinetin. Shoot and root formation were respectively optimized in MS+3.0 mg L–1 (BAP)+0.1 mg L–1 (NAA) and MS+1.0 mg L–1 (IBA)+0.1 mg L–1 2,4-D. The highest fresh weight of callus and increase in proline enzyme concentrations were obtained through acute gamma radiation at 7% PEG strength. Conclusion: This study successfully establishes a novel potential mutant line as an improved drought-tolerant MRQ74 variant. Further research should be conducted to investigate the nutrient properties and yield of this novel mutant.
Research Article
Published on October 15, 2016
Study on Technology Optimization of Lignin Removalin Cellulose Extraction from Wheat Bran by Combination of Ultrasound and Hydrogen Peroxide
Liu Jun-Hong and Wang Kun-Yu
Background: Currently, cellulose is more closely related to people’s daily life and widely used in various fields, textile industry, food, medicine and health care etc. Thus the increasing research focuses on cellulose extraction. Wheat bran, by-product of flour processing, is rich in cellulose, its cellulose extraction is conducive to the rational use of wheat bran. In wheat bran, cellulose is wrapped by lignin, semi-cellulose and pectin, which prevented it from high yield, therefore the removal of lignin, semi-cellulose and pectin benefits to the extraction efficiency. Materials and Methods: Taking wheat bran as raw material and using the method of ultrasonic assisted, the researchers studied the factors, affecting lignin removal, to find out the optimum conditions. Effects of hydrogen peroxide concentration, liquid-solid ratio, temperature, ultrasonic pretreatment power, reaction time on removal rate of lignin were analyzed respectively. On the basis of the above experiments of single factors, design orthogonal experiment to determine the optimum conditions of lignin removal. Results: Experimental results showed that removal rate of lignin reached 73.87% with the ratio of liquid to solid of 15:1, the ultrasonic treatment time of 10 h at 50°C, the power of 100 W and the hydrogen peroxide concentration of 3%. Conclusion: Traditionally, lignin removal was carried out by chemical reagent from straw of various kinds and wood chips, along with the environmental pollution. In this study, taking wheat bran as material by the combination of ultrasound and chemical reagent process, lignin was removed to a great degree, hence cellulose was extracted with high yield and chemical reagent experienced decreased consumption, which provided enough material for food industry, medicine industry and other areas.
Research Article
Published on October 15, 2016
Soild-state Fermentation of Coconut Coir by Pleurotus sajor-caju Increases the Anti-oxidant Properties and Nutritional Value
Hossain Mohammad Shamim, Md. Shakhawat Hussain and Abdullah- Al-Mahin
Background and Objective: An enormous amount of lignocellulosic materials are produced every year throughout the world which can be converted to nutritionally enriched ruminant feed by combination of chemical and biological treatment. This study was undertaken to improve nutritional values of lime treated coconut coir, a lignocellulosic material, by solid-state fermentation with Pleurotus sajor-caju. Methodology: The CaCO3 treated coconut coir was fermented with P. sajor-caju for 56 days at 30°C. Changes of crude protein, lipid, carbohydrate, ash, lignin, cellulose, hemicellulose, reducing sugar, ascorbic acid and carotinoid contents due to fermentation were detected using standard methods. Results: At the end of 56 days fermentation, crude protein, ash and reducing sugar contents were increased by 744.44, 55.61 and 113.80%, respectively. However, crude fiber, lipid, carbohydrate, lignin, cellulose and hemicelluloses contents were decreased by 33.04, 63.42, 20.94, 25.75, 18.57 and 24.42%, respectively. Ascorbic acid and carotenoid were increased by 63.18 and 580.49%, respectively. Conclusion: Thus, bioconversion of coconut coir by P. sajor-caju offers a promising way to convert the lignocellulosic wastes into nutritionally improved animal feed.

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